Guido Ambrosini

Prof. Dott.

Guido Ambrosini

Ginecologo

Svolge attività di Specialista:

Istruzione e formazione

Il Prof. Guido Ambrosini, nato a Pescara il 9 maggio 1967, ha conseguito:

  • Laurea in Medicina e Chirurgia presso l’Università degli Studi di Sassari nel Luglio del 1992, con il massimo dei voti e la lode.
  • Specializzazione in Ginecologia ed Ostetricia presso l’Università degli Studi di Sassari nell’Ottobre 1996 con il massimo dei voti e la lode.

Esperienza professionale

  • Ricercatore universitario S.S.D. F20X presso l’Università degli Studi di Sassari dal 12 Ottobre 1993.
  • Conferimento delle mansioni superiori di Aiuto ai fini assistenziali dal 1 Novembre 1995.
  • Stage per attività di ricerca presso la Monash University Institute of Reproduction and Development – Melbourne dal 01 febbraio 1997 al 31 gennaio 1998 .
  • Stage per attività di ricerca presso la New York University Medical Center, Program for In Vitro Fertilization, Reproductive Surgery and Infertility, NYC (USA) dal 01 ottobre 1998 al 30 settembre 1999.
  • Ricercatore Universitario Confermato presso il Dipartimento di Scienze Ginecologiche e della Riproduzione Umana dell’Università degli Studi di Padova dal 1 Novembre 2000.
  • Stage come medico frequentatore in missione scientifica presso il Centre Hospitalier Universitaire Vaudois (CHUV) di Losanna dal 1 settembre al 31 ottobre 2001.
  • Idoneità a Professore di II fascia S.S.D. MED/40 – Ginecologia e Ostetricia presso l’Università di Genova in data 11 Luglio 2003.
  • Professore Associato di Ginecologia ed Ostetricia S.S.D. MED/40 – Ginecologia e Ostetricia presso il Dipartimento di Scienze Ginecologiche e della Riproduzione Umana dell’Università degli Studi di Padova dal 1 Marzo 2006.
  • Docente nella Scuola di Ostetricia, nel Diploma Universitario di Ostetrica/o, nel Corso di Laurea in Ostetricia, nel Corso di Laurea in Biotecnologie Mediche, nel Corso di Laurea in Fisioterapia, nel Corso di Laurea in Infermieristica, nella Scuola di Specializzazione in Ginecologia e Ostetricia, nel Corso di Laurea Specialistica e poi Magistrale a Ciclo Unico in Medicina e Chirurgia nelle sedi di Venezia, Treviso e Padova.
  • Docente nel Dottorato di Ricerca in Medicina dello Sviluppo e Scienze della Programmazione Sanitaria dell’Università di Padova dal febbraio 2017.
  • Vice Direttore della Scuola di Specializzazione in Ginecologia e Ostetricia dal 31 gennaio 2007 al 14/12/2009.
  • Vice Presidente del Corso di Laurea in Ostetricia dal 1 Novembre 2003 al 14/02/2009.

Premi e riconoscimenti per l’attività scientifica

  • F1000 PRIME – Article Reccomendations
    Evaluation of correct endogenous reactive oxygen species content for human sperm capacitation and involvement of the NADPH oxidase system.
    Human Reproduction, 2011 Dec. 26 (12): 3264 – 3273
    Donà G., fiore C., Andrisani A., Ambrosini G., Brunati A., Ragazzi E., Armanini D., Bordin L. , Clari G.
    Reccomended by Maciej Kurpisz, Monika Fraczek and Marta Olszewska (13/01/2012).
  • International Award for publishing Excellence in The Journal of Clinical Endocrinology and Metabolism conferito dalla International Socity of Endocrinology (ISE) nel 16th International Congress of Endocrinology (ICE) – Chicago – USA, dal 21.06.2014 al 24.06.2014, per il lavoro: Human red blood cells alterations in primary aldosteronism. Bordin L., Donà G., Sabbadin C., Ragazzi E., Andrisani A., Ambrosini G., Brunati A.M., Clari G., Armanini D. pubblicato nel 2013 nel Volume 98 (6), 2494 – 2501.
  • Presentazioni selezionate dal Comitato Scientifico del Meeting Annuale dell’Associazione Ginecologi Universitari Italiani (AGUI) “Donna Mamma Feto” Milano 30 novembre – 1 dicembre 2017:
    • Utilizzo della Corifollitropina alfa nella gestione delle pazienti poor responder che si sottopongono a trattamenti di fecondazione in vitro. Marin L., Sabbadin C., Mosele S., Ambrosini G., Andrisani A.
    • L’influenza dell’autoimmunità tiroidea sulla qualità embrionaria nelle donne che si sottopongono a trattamenti di fecondazione assistita. Sabbadin C., Marin L., Mosele S., Ambrosini G., Andrisani A.
  • “Full Congress Package” conferito dal Comitato Scientifico del 18° World Congress Gynecological Endocrinology, Firenze 7 – 10 marzo 2018 per Abstract: “Effect of astaxanthin on human sperm capacitation: in vivo ed in vitro studies”. Donà G., Sabbadin C., Carraro C., Ragazzi E., Armanini D., Ambrosini G., Andrisani A., Bordin L.

Partecipazione a comitati editoriali di riviste

  • Membro del comitato editoriale della rivista “il Ginecologo” dal 14.03.2006 ad oggi.
  • Membro dell’Editorial Board della rivista internazionale: “Gynecology and Obstetrics International” dal 01.01.2014 ad oggi.
  • Membro dell’Editorial Board della rivista internazionale: “Case Reports in Obstetrics and Gynecology” dal 01.01.2014 ad oggi.

Pubblicazioni

  • A suggestion to overcome potential risks for infertility due to COVID-19: A response to Aitken

    Loris Marin, Guido Ambrosini, Ludovica Nuzzi, Luciana Bordin, Alessandra Andrisani

    We are writing this letter as scientists that contributed to leading peer reviewed journals regarding female and male fertility. In particular, our group investigated the possible effects of a natural carotenoid (Astaxanthin) on human spermatozoa. We have carefully read the article by Robert John Aitken regarding the role of SARS-CoV-2 on spermatozoa.

    The author points out that mature spermatozoa manage to bind the virus and also achieve reverse transcription. By doing this, spermatozoa could act as vectors for the disease. In fact, spermatozoa express ACE1 and ACE2 and the infection generates an increase of angiotensine-II. The ACE2 is important in sperm biology because it prevents spermatozoa from engaging the apoptotic pathway. The exposure to COVID 19 causes the cleavage of ACE inducing a decrease in sperm viability and function. Aitken also states that COVID attack increases angiotensin availability and this increases sperm fagocitosis by neutrophil and the local levels of reactive oxygen species (ROS). As evidenced in one of our studies and Aitken’s study, ROS production is required for sperm capacitation, however high levels of inflammation have a negative effect upon sperm4 and SARS-CoV-2 may impact on sperm quality also through this mechanism.

    A febrile episode can have adverse effects on conventional semen parameters (sperm volume, sperm count, vitality, motility, and morphology) as well as on sperm DNA integrity, but these are temporary and reversible. These effects may be brought about by two factors, hyperthermia and heat stress. There is evidence that hyperthermia can adversely affect sperm membrane integrity and fertilization capacity. In addition, oxidative stress, as a consequence of high temperature, may play a role in the suppression of spermatogenesis. We have previously demonstrated the role of Astaxanthin (Asta) on human semen. Asta is a photo-protective red pigment belonging to the carotenoid family that shares a similar beta-carotene structure. It is synthesized by the microalgae Haematococcus pluvialis in the presence of stressful conditions including, nitrogen deficiency, high salinity, and high temperature. Asta is known for its antioxidant and anti-inflammatory properties, and more specifically it inhibits cyclooxygenase 2 enzyme activities. It is present in many dietary supplements and is also used for sperm quality improvement treatment.

    We previously demonstrated that Asta has positive effects on some semen parameters and male fertility by increasing sperm concentration and linear velocity, thus ameliorating fertilizing power. Moreover, sperm undergoes a series of transformations during the process of capacitation, including ROS production and membrane rafts translocation, which are necessary for acrosome reaction. In particular, Asta is shown to ameliorate the Tyr-phosphorylation Tyr-P pattern of the head and acrosome reaction independently of the redox process of cells. It is possible to insert Asta into the membrane leading lipid rafts relocation and create a capacitation-like membrane alteration. Therefore, a subsequent acrosome reaction can take place.

    Aitken reports that a recent analysis revealed that among 38 semen samples from COVID 19 patients six were tested positive for the virus by RT-PCR and also with the presence of the virus in the seminal fluid Asta might play a role. In fact, it has been demonstrated that Asta can prevent the human papillomavirus L1 protein binding in human sperm membranes, reducing the presence of L1 by more than 50%.

    To conclude, as evaluated by Aitken, patients with SARS-CoV-2 infection may have altered semen, as the infection could potentially affect spermatogenesis through inflammation with an exaggerated production of ROS, while Asta supplementation could lead to reduced inflammation, keeping the essential levels of ROS required for sperm capacitation.

    2021

    PMID: 34415678 – PMCID: PMC8444684

  • Intrahepatic cholestasis of pregnancy after ovarian hyperstimulation syndrome with wild-type ABCB4 gene: a peculiar case and literature review

    Loris Marin, Guido Ambrosini, Ludovica Nuzzi, Giovanni Buzzaccarini, Federica Esposito, Giampiero Capobianco, Vito Chiantera, Antonio Simone Laganà & Alessandra Andrisani

    Intrahepatic cholestasis of pregnancy (ICP) in the first trimester occurring after ovarian hyperstimulation syndrome (OHSS) is a rare condition and few cases are reported in the literature. Hyperestrogenism may explain this problem in genetically predisposed women. The objective of this article is to report one of these rare cases and offer an overview of the other published cases.

    2023

    BMC Women’s Health volume 23, Article number: 316 (2023)

  • IVF Lite: a smart IVF programme based on mild ovarian stimulation for good prognosis patients

    Luca Gianaroli, Amerigo Vitagliano, Anna P Ferraretti, Silvia Azzena, Gaia Terzuoli, Davide Perruzza, Guido Ambrosini, Carla Tabanelli, M Cristina Magli

    The IVF Lite programme is based on mild ovarian stimulation including up to three fresh/frozen embryo transfers within 12 months. Is it effective and safe in good prognosis patients?

    2022

    45(2):256-263

  • Chronic endometritis and altered embryo implantation: a unified pathophysiological theory from a literature systematic review

    Giovanni Buzzaccarini, Amerigo Vitagliano, Alessandra Andrisani, Carla Mariaflavia Santarsiero, Rossana Cicinelli, Claudia Nardelli, Guido Ambrosini, Ettore Cicinelli

    Chronic endometritis (CE) is a frequent hysteroscopic and histological finding which affects embryo transfer implantation during IVF-ICSI cycles. In particular, CE impairs proper decidualization and, subsequently, implantation. Although this correlation has been clearly clarified, a pathophysiological explanation assembling all the studies performed has not been elucidated yet. For this reason, we have structured a systematic review considering all the original articles that evaluated a pathological element involved in CE and implantation impairment.

    2020

    37(12):2897-2911.

  • Middle Cerebral Artery Peak Systolic and Ductus Venosus Velocity Waveforms in the Hydropic Fetus

    Erich Cosmi, Salvatore Dessole, Luisa Uras, Giampiero Capobianco, Donato D’Antona, Alessandra Andrisani, Pietro Litta, Guido Ambrosini

    The purpose of this study was to assess whether Doppler assessment of the middle cerebral artery (MCA) peak systolic velocity (PSV) and ductus venosus (DV) velocity waveforms during sonography of hydropic fetuses may specify the cause of fetal hydrops.

    2005

    24(2):209-13

  • Role of Renin-Angiotensin-Aldosterone System and Cortisol in Endometriosis: A Preliminary Report

    Chiara Sabbadin, Carlo Saccardi, Alessandra Andrisani, Amerigo Vitagliano, Loris Marin, Eugenio Ragazzi, Luciana Bordin, Guido Ambrosini, Decio Armanini

    Endometriosis is a chronic inflammatory disease associated with pelvic pain, infertility, and increased cardiovascular risk. Recent studies suggest a possible role of aldosterone as a pro-inflammatory hormone in the pathogenesis of the disease. Cortisol is also an important mediator of stress reaction, but its role is controversial in endometriosis. The aim of this study was to evaluate aldosterone and cortisol levels and blood pressure values in women with endometriosis. We measured blood pressure, plasma aldosterone, renin, cortisol, and dehydroepiandrosterone sulfate (DHEAS) in 20 women with untreated minimal or mild pelvic endometriosis compared with 20 healthy controls matched for age and body mass index. Aldosterone values were similar in the two groups, while renin was significantly lower and the aldosterone to renin ratio was significantly higher in patients with endometriosis than in controls. Systolic blood pressure was in the normal range, but significantly higher in patients with endometriosis. Morning plasma cortisol was normal, but significantly lower in patients with endometriosis compared with controls, while DHEAS to cortisol ratio was similar in the two groups. These preliminary results are evidence of increased biological aldosterone activity and dysregulation of the hypothalamic-pituitary-adrenal axis in early stages of endometriosis. These alterations could play a role in disease development, suggesting new therapeutic targets for aldosterone receptor blockers.

    2022

    Int. J. Mol. Sci. – 24(1), 310

  • Uterine Septum with or without Hysteroscopic Metroplasty: Impact on Fertility and Obstetrical Outcomes-A Systematic Review and Meta-Analysis of Observational Research

    Marco Noventa, Giulia Spagnol, Matteo Marchetti, Carlo Saccardi, Giulio Bonaldo, Antonio Simone Laganà, Francesco Cavallin, Alessandra Andrisani, Guido Ambrosini, Salvatore Giovanni Vitale, Luis Alonso Pacheco, Sergio Haimovich, Attilio Di Spiezio Sardo, Jose Carugno, Marco Scioscia, Simone Garzon, Stefano Bettocchi, Giovanni Buzzaccarini, Roberto Tozzi, Amerigo Vitagliano

    We performed a systematic review/meta-analysis to evaluate the impact of septate uterus and hysteroscopic metroplasty on pregnancy rate-(PR), live birth rate-(LBR), spontaneous abortion-(SA) and preterm labor (PL) in infertile/recurrent miscarriage-(RM) patients. Data sources: a literature search of relevant papers was conducted using electronic bibliographic databases (Medline, Scopus, Embase, Science direct). Study eligibility criteria: we included in this meta-analysis all types of observational studies that evaluated the clinical impact of the uterine septum and its resection (hysteroscopic metroplasty) on reproductive and obstetrics outcomes. The population included were patients with a diagnosis of infertility or recurrent pregnancy loss. Study appraisal and synthesis methods: outcomes were evaluated according to three subgroups: (i) Women with untreated uterine septum versus women without septum (controls); (ii) Women with treated uterine septum versus women with untreated septum (controls); (iii) Women before and after septum removal. Odds ratios (OR) with 95% confidence intervals (CI) were calculated for the outcome measures. A p-value < 0.05 was considered statistically significant. Subgroup analysis was performed according to the depth of the septum. Sources of heterogeneity were explored by meta-regression analysis according to specific features: assisted reproductive technology/spontaneous conception, study design and quality of papers included Results: data from 38 studies were extracted. (i) septum versus no septum: a lower PR and LBR were associated with septate uterus vs. controls (OR 0.45, 95% CI 0.27−0.76; p < 0.0001; and OR 0.21, 95% CI 0.12−0.39; p < 0.0001); a higher proportion of SA and PL was associated with septate uterus vs. controls (OR 4.29, 95% CI 2.90−6.36; p < 0.0001; OR 2.56, 95% CI 1.52−4.31; p = 0.0004). (ii) treated versus untreated septum: PR and PL were not different in removed vs. unremoved septum(OR 1.10, 95% CI 0.49−2.49; p = 0.82 and OR 0.81, 95% CI 0.35−1.86; p = 0.62); a lower proportion of SA was associated with removed vs. unremoved septum (OR 0.47, 95% CI 0.21−1.04; p = 0.001); (iii) before-after septum removal: the proportion of LBR was higher after the removal of septum (OR 49.58, 95% CI 29.93−82.13; p < 0.0001) and the proportion of SA and PL was lower after the removal of the septum (OR 0.02, 95% CI 0.02−0.04; p < 0.000 and OR 0.05, 95% CI 0.03−0.08; p < 0.0001) Conclusions: the results show the detrimental effect of the uterine septum on PR, LBR, SA and PL. Its treatment reduces the rate of SA.

    2022

    J. Clin. Med. – 11(12), 3290

  • Hysteroscopic metroplasty under laparoscopic guidance in infertile women with septate uteri: follow-up of reproductive outcome

    Pietro Litta, Chiara Pozzan, Federica Merlin, Giuseppe Sacco, Carlo Saccardi, Guido Ambrosini, Giampiero Capobianco, Salvatore Dessole

    To evaluate the effectiveness of hysteroscopic metroplasty under laparoscopic guidance in the treatment of infertile women with a diagnosis of septate uterus and the impact of this surgical procedure on reproductive outcome.

    2004

    J Reprod Med – 49(4):274-8.